Processing and function of undermethylated chicken embryo fibroblast mRNA.
نویسندگان
چکیده
منابع مشابه
Pock forming ability of fowl pox virus isolated from layer chicken and its adaptation in chicken embryo fibroblast cell culture.
AIM The objective of the present study was to examine pock forming ability of field strain and vaccine strain of fowl pox virus (FPV) in chorioallantoic membrane (CAM) of embryonated chicken eggs and its adaptation in chicken embryo fibroblast (CEF) cell culture. MATERIALS AND METHODS Dry scabs were collected from 25 affected birds in glycerin-saline and preserved at 4°C until processed. Viru...
متن کاملMolecular diagnosis and adaptation of highly virulent infectious bursal disease virus on chicken embryo fibroblast cell
Materials and Methods Infectious bursal disease (IBD) is a highly contagious disease of young chicken caused by infectious bursal disease virus (IBDV), characterized by immunosuppression and mortality generally at 3 to 6 weeks of age [1]. It is economically important to the poultry industry worldwide, due to increased susceptibility to other diseases and negative interference with effective vac...
متن کاملMicroarray analysis of early and late passage chicken embryo fibroblast cells.
Primary cultured cells derived from normal tissue have a limited lifespan due to replicative senescence and show distinct phenotypes such as irreversible cell cycle arrest and enlarged morphology. Studying senescence-associated genetic alterations in chicken cells will provide valuable knowledge of cellular growth characteristics, when compared with normal and rapidly growing cell lines. Microa...
متن کاملTranscriptome Analysis of Chicken Embryo Fibroblast Cell Infected with Marek’s Disease Virus of GX0101∆LTR
Marek’s disease (MD), a lymphoproliferative disorder of chickens caused by the MD virus (MDV), is economically significant. The resistance/susceptibility to MD is controlled by host genetics. The host response to different virus strains varies. The pathogenicity of REV-LTR deleted GX0101∆LTR MDV has been previously reported. However, the precise molecular mechanism of the response of chickens t...
متن کاملHuman cytomegalovirus induces alteration of beta-actin mRNA and microfilaments in human embryo fibroblast cells.
OBJECTIVE To investigate the infection of human embryo fibroblast cell line HF cells by CMV as well as the effects of CMV on beta-actin mRNA and microfilaments. METHODS HF cells shape was observed after the infection of CMV. RT-PCR assay was used to detect the mRNA expression of CMV immediate early (IE) gene, beta-actin and GAPDH genes of HF cells infected by CMV. CMV particles and cell micro...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1979
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)50451-8